Over the course of the semester, our trainees are reviewing webinars in their given fields and preparing abstracts to help colleagues outside their discipline make an informed choice about watching them. As our program bridges diverse disciplines, these abstracts are beneficial for our own group in helping one another gain key knowledge in each other’s fields. We are happy to share these here for anyone else who may find them helpful.
Highly Multiplexed Targeted Proteomics Acquisition on a TIM-Q-TOF
Prof. Dr Gunnar Dittmar, & Dr. Jarrod A. Marto
April 28, 2021
Hosted by Wiley Analytical / Bruker Dal tonics
Watch the Webinar (Requires free registration through Wiley Analytical)
Analysis by Samuel Okyem:
Differential expression of proteins in disease conditions are mostly monitored by measuring the global or spatial transcriptome. However, not all expressed RNA are converted to protein products. To correlate gene expression to protein product, it is essential to develop effective quantitative protein measurement tools. Targeted proteomics is an efficient analytical technique for measuring specific proteins or peptides in complex biological samples. The technique allows the identification of proteins, serving as disease biomarkers or those that are differentially expressed in a certain conditions or experimental controls. Additionally, protein measurement can be used to monitor the progression or disease onset.
This webinar unveils the potential application of trap ion mobility quadrupole time of flight (TIMS-Q-TOF) mass spectrometry in quantitative proteomics. The label free proteomic technique eliminates the use of expensive tags and isotopic labels in quantitative proteomics, therefore making it a cost-effective and sensitive peptide quantitating tool. TIMS-Q-TOF leverages the ability of trap ion mobility to simultaneously accumulate and release ions of interest. This technology removes interfering ions as well as increasing reproducibility, which is not usually obtained in a general data dependent acquisition mass spectrometry.
The effectiveness of this analytical technology was proven by measuring almost a hundred and twenty isotopically labelled peptides spiked into a tryptic Hela cell digest and the levels of mutant KRAS, NRAS and HRAS oncogenes. The method can be used to measure differential levels of neuropeptides and proteins which can facilitate understanding neurological processes.
Although the presenters did a great job in explaining the technology and some of its relevant applications, they were very technical, making it not suitable for a broader audience. Nonetheless, the webinar is very useful for analytical and protein scientists.